ABSTRACT
Capillary electrophoresis-mass spectrometry is a powerful analytical technique that combines high efficiency, short analysis time, high selectivity, and separation versatility of the CE with the selectivity, sensitivity, and possibility for identification and structure elucidation of unknown analytes from the MS detection point of view. CE is one of the most frequently used techniques for the separation of optically active compounds. The major mechanism applied in the enantioseparation by CE is based on the formation of diastereomeric complexes between the separated enantiomers and the chiral selector in background electrolyte during the separation process. Sheathless interface is largely handled by a metal coating of separation capillary tip with the inner diameter of 100 gm and thus provides a conductive electrical connection, which is necessary for the particular separation in CE and electrospray ionization (ESI). Since the capillary flows of the electrolyte are about 100 times lower, it is possible to increase the flow to the desired rate by adding an auxiliary liquid.
