ABSTRACT
Lasers can deliver high power and coherent radiation. The features make them ideal for applications in different types of optical microscopy. Transient absorption is the secondary absorption of specific molecules that are already excited by the primary absorption. The differences between transient absorption microscopy and classical imaging and microscopy lie in their high temporal resolution and in their ability to study non-fluorescent molecular species. Therefore, this method belongs to the group of label-free imaging methods. The coherence of laser radiation enables coherent diffraction imaging, which is also called ‘lensless microscopy’. When the object is illuminated by the coherent radiation, scattered light produces a diffraction pattern containing information of the object.
