ABSTRACT
The identification and quantitative analysis of opioids must employ specific methods, primarily including chromatographic methods combined with various types of detectors. Despite the significant role of preliminary examinations in forensic toxicology, they are insufficient to identify new opioids. No cross reactions to any novel opioid-like substances have been reported in the case of regularly used preliminary methods, such as enzyme-linked immunosorbent assay, enzyme-multiplied immunoassay technique, or immunochromatographic rapid tests for drugs of abuse, detecting morphine, its metabolites, and other opiates. The first analytical data on the detection of novel opioids, as in the case of AH-7921, inform of the use of known and commonly used methods of biological sample extractions. It is important that all the discussed methods include the use of separation methods for the isolation of the targeted compounds from the biological and nonbiological samples.
