The present study was conducted to explore the hidden potential of natural products synthesized in the medicinal plant Tinospora cordifolia. This plant is prioritized by National Medicinal Plant Board, New Delhi. Leaf and internodal segments were inoculated on Murashige and Skoog medium fortified with indole-3-butyric acid (1.0 mg/L) produced callus after 4 weeks. The calli were brown due to phenolic substance secreted by the explant. This problem was overcome by using adjuvant activated charcoal (3.0%). Further, we are trying to isolate berberine and tinosporin alkaloids from the callus and enhance the amount of these alkaloids through callus culture.