ABSTRACT
The synchronous heartbeat is the best and most cited example of gap junctions' electrical function. Junctional membranes represent a special case of semipermeable membrane through which the prospective separation of charges would occur between two virtually identical compartments. Junctional permeability is determined by the connexin isotype(s) composing the channels. Since not all connexins form highly permeable gap junction channels, the molecular size limit for junctional diffusion must be smaller in some tissues than in others. Stable connexin-expressing cells are obtained after transfection, using cell dilution and specific antibiotic selection of connexin-positive cell clones. An electrical rack cabinet or tower is a sturdy metallic structure to hold all electronics in a tiered order and to facilitate connections and relocation. Imperfectly chlorided or scratched wires can cause electrical noise and baseline drift. Changes on the chamber solution level that decrease the immersion of the ground wire may also cause baseline drifts.
