ABSTRACT

The extreme pursuit of economic success by over management and unreasonable development has profoundly exploited natural resources and damaged environmental ecosystems. Nature has its own ways of resolving imbalances of its environment and microorganisms often serve as drivers that work effectively to eliminate toxic pollutants. Metagenomics provides information about immense diversity and the functional ability of microorganisms inhabiting polluted samples. bioremediation has been considered as one of the potential tools to remove contaminants from the environment. Molecular ecologists are analyzing environmental deoxyribonucleic acid from diverse ecosystems such as soil, water, etc., which allows access to genomes of uncultivable microorganisms. Microbial fingerprinting methods are often employed to differentiate organisms or classes of organisms based on unique character of a universal component or section of a biomolecule. The role of microbial diversity and functions with respect to bioremediation of diesel-contaminated soils was assessed using both metagenomic analyses and enzymatic assays.