ABSTRACT
The spectrophotometric assay of catalase, as originally developed by Beers and Sizer 1 in 1952, has been employed in the purification of catalases from both prokaryotic 2 , 3 and eukaryotic 4 sources. Each of these tetrameric enzymes contains 1 mol of a tightly bound heme prosthetic group per subunit, and the enzyme catalyzes the decomposition of hydrogen peroxide as follows:
