ABSTRACT
The main advantage of the stain is that it shows the mucus yellow, and Helicobacter pylori as purple red curved bacteria, often visualized within the mucus layer. In a direct comparison between Giemsa, cresyl fast violet, modified Dieterle, Gimcnez, and the carbol fuchsin stains, both Giemsa and carbol fuchsin were found to be suitable stains for routine identification of H. pylori. Their use can be advocated if familiarity with the techniques exists, but in many instances a pathologist well trained in Helicobacter pyloridiagnosis may do just as well examining regular histology stains. Pyloriset is a latex agglutination test that uses latex particles coated with acid-extracted antigen of H. pylori. Molecular tests offer excellent means of very precise diagnosis of H. pylori infection. The first reports of Polymerase Chain Reaction for diagnosis of H. pylori infection used primers that amplified a fragment of the bacterial ribosomal 16S genes. There is a wide variety of diagnostic tests available for H. pylori.
