ABSTRACT
In this chapter, the authors describe the nucleotide sequence of the Autographa californica nuclear polyhedrosis virus (AcNPV) homologous regions and present evidence that they function as enhancers of delayed early gene transcription. Expression of viral genes in AcNPV-infected cells is temporally regulated. The genomic location of an immediate early gene (IE-1) was functionally mapped using a series of deletions of a plasmid containing the EcoRI-B fragment of AcNPV DNA. The authors examine the homologous regions which are rich in EcoRI sites were involved in 39CAT expression. The difference between cotransfection of pEcoRI-B, which stimulated 39CAT, and EcoRI- digested DNA, which did not stimulate 39CAT, was attributed to a combination of two factors, the concentration and the conformation of the plasmid DNA. The complete sequence of the homologous regions has been presented by L. A. Guarino et al. Homologous sequences extending to the left and right of the first and last EcoRI sites are also included.
