ABSTRACT
This chapter describes the methodological aspects of the application of perturbed angular correlation (PAC) for the study of the fate of liposomes in vivo. In principle, any gamma-emitting nucleus whose nuclear spin in the intermediate state of the cascadc is greater than, or equal to one, can be used as a candidate for the PAC study. The technical aspect of the application of PAC for studying the stability of liposomes is very simple. In addition to studying the degradation of liposomes in vivo by the approach, it is also possible to monitor the liver degradation of liposomes in vitro using an isolated liver. Extreme care should be taken to ensure that the rate of degradation of liposomes in the liver obtained under in vitro simulated conditions is similar to the in vivo rate. The use of the PAC technique to study the structural integrity of liposomes has broad applicability in vitro as well as in vivo.
