ABSTRACT
This chapter describes hybridomas to herpes simplex viruses (HSV) 1 and 2 and the use of monoclonal antibodies as serologic reagents to study antigenic determinants of the viral glycoproteins. It produces hybridomas and selected for antibody with neutralizing and nonneutralizing activity to HSV-1 and HSV-2. Viral glycoproteins bound to intracellular membranes and inserted into the plasma membrane of infected cells share antigenic determinants with glycoproteins in the virion envelope. The denatured gC glycoprotein retained many type specific determinants since antisera produced in this way discriminated between HSV-1 and HSV-2 in neutralization tests and reacted by immunofluorescence and agglutination only with homologous virus. Immune precipitation tests were done to characterize the reactivity of monoclonal antibody to HSV glycoproteins. The chapter shows that the genes for glycoproteins gA and gB are located between 0.03 and 0.42 map units on HSV genome. Hybridoma antibody H233 precipitated glycoproteins gA and gB and hybridoma antibody HD1 precipitated glycoprotein gD from cells infected with both serotypes.
