ABSTRACT
This chapter describes the techniques required for obtaining high-resolution nuclear magnetic resonance (NMR) spectra from solid and semisolid samples. The last decade has brought a substantial growth in the application of NMR techniques to biochemical problems. The techniques presented in this chapter are capable of yielding "high-resolution" solid state NMR spectra. When the sample investigated is a single crystal, well-resolved line spectra are observed and the angular dependence of the line positions can be examined to determine chemical shift tensors, and quadrupole tensors. Magic angle sample spinning (MASS) is often employed to regain "high-resolution" spectra of powder samples. MASS can be combined with both multiple-pulse and dilute spin double resonance for further spectral enhancements. Each crystallographically inequivalent nucleus will yield one line for a given crystal orientation. Chronologically, MASS was the first technique suggested for obtaining high-resolution spectra from solids, and is quite simple in principle.
