ABSTRACT
Solid-phase methods address the problem of sample washout by covalent immobilization of the polypeptide to a solid support, thereby eliminating the problem of removal of excess reagents or recovery of the 3-phenyl-2-thiohydantoin-amino acid from the remainder of the polypeptide. There are several recent reviews of solid-phase sequence analysis emphasizing microsequencing and extended sequence runs. The relevance of some aspects of micropurification methods to solid-phase microsequence analysis has been described in a recent review. At the present time, there are three preferred methods of polypeptide immobilization for solid-phase microsequence analysis. A method for covalent electrotransfer has been recently developed which promises to become a powerful addition to the covalent immobilization methods available for solid-phase sequence analysis. Several of the important advantages of solid-phase microsequence analysis manifest themselves when microanalytical method is used in conjunction with the previously described micropurification methods.
