ABSTRACT
This chapter contains an analysis of some of the recombinant DNA techniques that have been employed for the manipulation of foreign genes in microorganisms resulting in protein production as diagrammed. The cloning and expression of foreign genes using recombinant DNA technology has permitted access to complex biological mechanisms such as eucaryotic RNA splicing, oncogene dynamics, and developmental systems such as antibody diversity. In addition, the technology has been the foundation for a new bio-technology industry. The isolation and cloning of genes involves a series of linked enzymatic steps. Isolation of clones containing cDNA made against very low abundance mRNAs depends on the generation of a sufficient number of clones according to the criteria described above and a strong screening procedure which will be described later. One of the major uses of the DNA polymerase 1 is nick-translation labeling of DNA fragments.
