ABSTRACT
This chapter illustrates the radical changes in the levels of containment requirements for using eukaryotic virus vectors and for cloning of viral DNA in different revisions of the National Institutes of Health (NIH) Guidelines. The suggestions of a working group on eukaryotic viral vectors of the Recombinant DNA Advisory Committee on April 13, 1980, at the American Society for Microbiology meeting in Miami Beach, Florida were also a basis for a significant change in the Guidelines. The containment conditions specified by the first NIH Recombinant DNA Guidelines promulgated in 1976 for experiments with animal viruses were extremely stringent. In addition, the suggestions of a working group on eukaryotic viral vectors of the Recombinant DNA Advisory Committee on April 13, 1980, at the American Society for Microbiology meeting in Miami Beach, Florida were also a basis for a significant change in the Guidelines.
