Replication of the Poliovirus Genome
DOI link for Replication of the Poliovirus Genome
Replication of the Poliovirus Genome book
The picornaviruses represent an important group of eukaryotic pathogens, the intracellular life cycles of which are characterized by several unique macromolecular/biosynthetic events. Poliovirus is a member of the enterovirus group of the Picornaviridae and perhaps best exemplifies the unique aspects of the Picornavirus group. The coding region of the polio genome contains a single, long, open-reading frame that encodes 2209 consecutive amino acids that would give rise to a polyprotein having a molecular weight of 247,000 daltons. The overall genome structures and polyprotein organization of all picornaviruses are roughly equivalent. The viral RNAs uniformly possess a 5'-linked VPg protein and a 3' polyadenylate tract. The crude and partially purified RNA replication complexes from extracts of virus-infected HeLa cells are associated with numerous host-cell proteins. Poliovirus RNA synthesis occurs primarily in a membranous environment. In vitro RNA synthesis assays using a crude membrane fraction from infected cells demonstrated that the membrane fraction is responsible for the majority of virus-specific RNA synthesis.