ABSTRACT
Cell adhesion is a ubiquitous process that influences many aspects of cell behavior. At low concentrations of hyaluroic acid, the collagen fibers were not fully coated, and areas of both hyaluroic acid and collagen were available for cell adhesion, either directly or through any mediating protein. Vitronectin (VN) is of interest because of its roles in cell adhesion and spreading, in coagulation, in complement activation, and in three important aspects of artificial surface induced thrombosis. The RGD-BSA conjugates were used to coat tissue culture plastic surfaces which then served as substrata in cell adhesion experiments. The surfaces of solvent-cast films of this polymer were analyzed by X-ray photoelectron spectroscopy by which surface chemical composition was quantitatively measured at the outermost layer of several tenths of angstroms, which determines biological responses including cell adhesion and growth.
