ABSTRACT
This chapter discusses that Saccharomyces cerevisiae is taken as the model because of the preponderance of data on this species. These are: timing, placement, and orientation. The molecular biology of budding is inherently interesting in terms of the underlying mechanisms which attend three dimensional changes in the cell envelope. Elucidation of budding mechanisms can be approached with a variety of techniques which are primarily biochemical and genetic in scope. Yeast budding is a suitable model system amenable to the various approaches needed to gain insight into cellular morphogenesis generally. The rate of mass increase limits progress toward a start signal that is required for bud emergence, DNA synthesis, and commitment to a cell cycle. The length of time needed to reach the mass required for bud emergence accounts for most of the effects of nutritional conditions on the rate of budding and division.
