ABSTRACT
However, the leucine mutant showed substantial loss of stereospecificity of isoproterenol in receptor binding studies, and in the activation of adenylate cyclase after stable expression of the leucine mutant in CHO cells. Intriguingly, the loss of affinity on the leucine mutant in a series of agonists appeared strongly correlated with the intrinsic activity of the compounds. Stereospecific recognition of antagonists such as propranolol was unaltered in this mutant (30).
