ABSTRACT
The major focus of the basic research of A. oryzae has been the analysis of hydrolytic enzymes, which are important players in fermentation. This includes purifi cation, characterization, and amino acid sequence analysis of various enzymes of interest. Cloning of the corresponding genes facilitated the analysis. However, existence of multiple conidia in a single cell makes it diffi cult to obtain mutants and lack of sexual generation in life cycle of A. oryzae prevents A. oryzae from being analyzed by traditional genetics.
