ABSTRACT
CONTENTS 23.1 Choice of Animal .................................................................................................................... 402
23.1.1 Murine Models .......................................................................................................... 402 23.1.2 Nonmurine Models ................................................................................................... 403
23.2 Immune Suppression ............................................................................................................... 404 23.2.1 Neutrophil-Based Immunosuppression .................................................................... 405 23.2.2 Macrophage-Based Immunosuppression ................................................................. 405
23.3 Fungal Inoculum Size ............................................................................................................. 406 23.4 Choice of Inoculation Route ................................................................................................... 406
23.4.1 Intranasal .................................................................................................................. 406 23.4.2 Intratracheal .............................................................................................................. 406 23.4.3 Inhalational ............................................................................................................... 407 23.4.4 Intravenous ................................................................................................................ 407 23.4.5 Cerebral .................................................................................................................... 408 23.4.6 Noninvasive to Invasive Disease .............................................................................. 408
23.5 Measurement of Outcome ....................................................................................................... 408 23.6 Conclusion ............................................................................................................................... 409 References .......................................................................................................................................... 410
The hallmark of translational research is the ability to transfer discoveries from the laboratory to the patient, and the crucial link in this marriage is the animal models that successfully mimic the human condition studied. Invasive aspergillosis (IA) is a complicated process requiring more than a single approach to accurately decipher and understand the pathobiology of both the fungus and the host. While there have been great advances in understanding genomics and proteomics in the last decade, the true application in medical mycology progresses through effective animal model testing.
