ABSTRACT
CONTENTS 29.1 Introduction ............................................................................................................................. 483 29.2 Comparison of Batch and Chemostat Fermentations ............................................................. 483 29.3 Microarrays and Filamentous Fungi ....................................................................................... 487 29.4 Design of Experiments ............................................................................................................ 487 29.5 Conclusions ............................................................................................................................. 489 References .......................................................................................................................................... 489
29.1 Introduction The recent availability of full genome sequences for some fi lamentous fungal species, for example, the human pathogen Aspergillus fumigatus (Nierman et al., 2005), the commercially important species A. oryzae (Machida et al., 2005) and Trichoderma reesei (https://www.genomesonline.org/), the model organism A. nidulans (Galagan et al., 2005) and the white rot fungus Phanerochaete chrysosporium (Martinez et al., 2004) (for a full list of all completed genomes and genomes being sequenced see https:// www.genomesonline.org/), together with the development of cutting-edge techno logies has led to the possibility of investigating global protein, mRNA and metabolite profi les of a chosen organism under certain defi ned growth conditions, as a result of a specifi c treatment or in response to an engineered change in the expression of a single gene. These technologies provide the possibility of characterizing cell physiology at a molecular level, providing temporal, spatial, and even real-time information (Hoskisson and Hobbs, 2005). However, these technologies require the production of reproducible, reliable and homogeneous datasets in order to gather meaningful information. The cell populations must, therefore, be grown in defi ned, ideally constant, controllable, physico-chemical conditions.
