ABSTRACT

Introduction ...................................................................................................................................... 31 Designing an Enrichment Medium .................................................................................................. 31

Energy Source ............................................................................................................ 32 Source of Carbon ....................................................................................................... 32 Trace and Major Elements ......................................................................................... 32 pH.......... ...................................................................................................................... 32 Physical Components ................................................................................................. 32 Antimicrobial Agents ................................................................................................. 32

Preservation of Microorganisms ...................................................................................................... 33 Introduction .......................................................................................................................... 33 Methods ................................................................................................................................ 33

Serial Subculture ........................................................................................................ 33 Storage at Low Temperatures .................................................................................... 33 Freeze-Drying ............................................................................................................ 33 Storage in Distilled Water ..........................................................................................34 Drying ......................................................................................................................34