ABSTRACT

I. Introduction — An Overview of Mammalian Glycosylation ............................1 II. Carbohydrate Biosynthesis ................................................................................3

A. Cellular Intake of Sugars ..........................................................................3 B. Intracellular Monosaccharide Processing ................................................11 C. Nucleotide Sugar Transport into the ER and Golgi ................................16 D. Glycoconjugate Biosynthesis: Glycoproteins, Glycolipids, and

Polysaccharides ........................................................................................16 1. O-Linked Glycosylation ..................................................................17 2. N-Linked Glycosylation....................................................................19