ABSTRACT
The colloidal silver technique recognizes non-histone proteins associated with specific loops of DNA called nucleolar organizer regions which encode ribosomal RNA. The advantages of techniques may be combined and the disadvantages avoided by cutting frozen sections and plastic-embedded sections from the same tissue block. By locating osteocalcin in osteoblasts and young osteocytes, the immunohistochemical technique has validated the clinical measurement of osteocalcin in blood as a routine biochemical marker of bone formation, frequently eliminating the need for a bone biopsy. The term electron probe X-ray microanalysis encompasses a number of techniques, often with slightly confusing names and acronyms. The technique provides a three-dimensional image that complements two-dimensional histology and has been used at low power for the examination of hard tissues, primarily to demonstrate the three-dimensional structure of cancellous bone in terms of plates and rods.
