ABSTRACT
In the USA, there are no means to visualize amyloid deposits radiographically in patients with primary (AL) amyloidosis. The specific interaction of the 11-1F4 mAb with AL amyloid fibrils presents a unique opportunity for its application as a non-invasive diagnostic and prognostic imaging tool for patients with this invariably fatal and devastating form of amyloid disease. MicroSPECT and Computerized tomography (CT) images were co-registered manually. Each mouse image carried fiducial markers supplied by capillaries filled with a solution of 125I which appeared in the SPECT and CT images and could be co-registered in the 3-D reconstructions. CT data were acquired using a MicroCAT™ II — this circular orbit conebeam system is equipped with a 20–80 kVp microfocus x-ray source.
