ABSTRACT
Barbadin microglobulin (ß2M) is well known as the precursor protein of dialysis-related amyloidosis and several variants of ß2M have been reported for hemodialysis patients since F. Gejyo’s report in 1985. ß2M consists of 99 amino acids and the monoclonal antibody for C-terminal 92-99 residues has been reported by M. Stoppini et al. to inhibit fibril formation in vitro. Seven carpal tissues were positive for Congo red and other 10 tissue specimens were negative for Congo red stain. All of the Congophilic areas showed a distinct staining for mAb92-99 and, conversely, no staining could be found in non-Congophilic areas as shown by arrows in the picture. The peptide identical to the C-terminal octa peptide was synthesized by an automated machine and conjugated with KLH. Then, injected into Balb/c mice and finally the monoclonal antibody was prepared according to Galfre/Milstein’s method.
