ABSTRACT
This chapter examines whether a monoclonal antibody specific to human Apolipoprotein E (apoE) fragments is bound to and is accumulated in AA amyloid deposits in vivo in human apolipoprotein E knock-in mouse with AA-amyloidosis. The antibody was iodinated with I for the accumulation or clearance study and was biotinylated for the tissue study. AA amyloidosis was induced by a single intraperitoneal administration of amyloid enhancing and 3 times intraperitoneal injections of 0.5 ml Fruend complete adjuvant with a week interval. The presence of the biotinylated antibodies in the amyloid tissues were studied histochemically using peroxidase-conjugated avidin. The antibodies accumulated in all the examined organs of amyloidotic mice with significant differences from non-amyloidotic mice at 72hrs after injection. Plasma clearance of the antibody in amyloidotic mice was similar to that of non-amyloidtic mice. The histological examination revealed that the injected antibodies were localized in the amyloid deposits in the spleen of the amyloidotic mice.
