ABSTRACT
Co-Expressed Purinergic Receptors .................................232 9.2.4 BRET Analysis.................................................................................233
9.2.4.1 BRET of Co-Expressed Purinergic Receptors .................234 9.2.4.2 Plasmid Constructs, Transfection, and BRET
Assay .....235 Acknowledgments..................................................................................................238 Abbreviations .........................................................................................................238 References..............................................................................................................238
It is well recognized that protein-protein interactions are fundamental processes for many biological systems between not only cytosolic proteins but also membranebound signaling proteins in the cell. In particular, the existence of homo-and heterooligomers between G protein-coupled receptors (GPCRs) that show distinct pharmacological and functional properties has been demonstrated.
