ABSTRACT
I. Extraction of Total RNA from Animals ...............................................................................26 A. Acid Guanidinium Thiocyanate Method ..................................................................26 B. LiCl/Urea Method .....................................................................................................27 C. Guanidinium Thiocyanate-CsCl Gradient Method ..................................................28 D. Phenol-Chloroform/LiCl Method..............................................................................29
II. Extraction of Total RNA from Plants ...................................................................................29 A. Alkaline Tris/Sarkosyl Method .................................................................................29 B. CTAB Method ...........................................................................................................31 C. Commercial RNA Extraction Kits ............................................................................31
III. Purification of Poly(A)
