ABSTRACT
I. Preparation of DNA Samples................................................................................................86 A. Restriction Enzyme Digestion of Genomic DNA ....................................................86 B. Agarose Gel Electrophoresis of DNA ......................................................................87
II. Blotting DNA onto Nylon Membranes ................................................................................89 A. Capillary Transfer Method ........................................................................................89 B. Alkaline Transfer Method .........................................................................................91 C. Vacuum Transfer Method..........................................................................................91
III. Preparation of DNA Dot/Slot Blots......................................................................................92 IV. Hybridization Procedures......................................................................................................94
