ABSTRACT
Contents 22.1 Introduction ..................................................................................................................385 22.2 Sample Preparation ...................................................................................................... 388
22.2.1 Free Essential Amino Acids ............................................................................ 388 22.2.2 Total Essential Amino Acids .......................................................................... 388
22.2.2.1 Acid Hydrolysis ............................................................................ 388 22.2.2.2 Alkaline Hydrolysis ...................................................................... 390 22.2.2.3 Enzymatic Hydrolysis ................................................................... 390
22.3 Analysis ........................................................................................................................391 22.3.1 Direct Spectrophotometric Methods ...............................................................391 22.3.2 Chromatographic Methods .............................................................................391
