ABSTRACT
Ligand Substitution Deactivation ............................................................................... 10 1.3.4 Nutrient Zn2+ De ciency and Apo-MT: Implications for Zn2+ Traf cking ................ 11 1.3.5 Utilization of Metal Binding Ligands to Induce Intracellular
Metal Ion De ciency ..........................................................................................13 1.3.6 Pharmacologic Ligands that Compete for Toxic Metal Ions ...................................... 15 1.3.7 Metallothionein and Intracellular Competition for Toxic Metal Ions ........................ 15
1.4 Metal Ion Exchange Chemistry .............................................................................................. 16 1.4.1 Zn-Proteins that Undergo Metal Ion Exchange with Cd2+: Metallothionein .............. 16 1.4.2 Zn-Proteins that Undergo Metal Ion Exchange with Cd2+: Zn-Finger Proteins ......... 18
1.5 Adduct Formation ...................................................................................................................20 1.5.1 Fluorescent Zn2+ Sensors in Relation to Zn2+ Traf cking ..........................................20 1.5.2 Fluorescent Zn2+ Sensor TSQ ..................................................................................... 21 1.5.3 What is TSQ Imaging in Cells? .................................................................................. 21
1.6 Redox Reactions .....................................................................................................................22 1.6.1 Iron Traf cking and Pharmaceutical Ligands for Fe Overload Disorder ...................22 1.6.2 Zn-Metallothionein and Sulfhydryl Group Redox Chemistry ...................................24
