ABSTRACT
An important goal in numerous chromatographic applications is to improve the separation power, P (Equation 9.1) or the speed of the separation [1]:
P(s−1) = dN dt
. (9.1)
This improvement is especially important in routine analyses and in largescale evaluation studies, such as the combinatorial characterization of drug candidates. High temperature (HT), HT high-performance liquid chromatography (HT-HPLC), and supercritical fluid chromatography (SFC) are common methods considered when separation speed is essential due the increased mass transport and decreased viscosity obtained with both techniques [2]. However, both SFC and HT-HPLC have deficiencies that limit their applicability [2,3]. For example, carbon dioxide, the most commonly-used solvent in SFC, has limited solvent strength even with the addition of small quantities of polar modifiers. The increased temperatures used in HT-HPLC can cause on-column reactions of the analytes [4]. Herein, we focus on enhanced-fluidity liquid chromatography (EFLC) that shares many of the positive attributes of each of these techniques without the negative attributes.
