ABSTRACT
In the years since its cloning, the sequence encoding the Aequorea GFP has been engineered to yield new FPs emitting light from the blue to yellowish green range of the visible spectrum (Tsien 1998; Cubitt, Woollenweber, and Heim 1999; Nagai et al. 2002; Rizzo et al. 2004; Ai et al. 2007). Furthermore, it is now well appreciated that many marine organisms produce FPs that are homologous to the Aequorea GFP (Matz, Lukyanov, and Lukyanov 2002; Labas et al. 2002; Shagin et al. 2004). Recently, some of the GFP-like proteins that are responsible for the bright colors we see in reef corals have become available for live-cell imaging applications (Matz et al. 1999; Karasawa et al. 2004; Shcherbo et al. 2007).
