ABSTRACT
Chemiluminescence (CL) is usually generated by fast reactions and hence the phenomenon can be followed only when the chemical reaction is initiated in front of the light detector. A plethora of analytical research on CL was carried out with modified fluorimeters [1] that allowed mixing of reagents and injection of the analyte through a syringe maintaining the lighttightness of the unit. The airsegmented continuous-flow system is not appropriate for CL measurements since the time between mixing of the reagents and passing the final solution through the debubbler before measurement is sometimes longer than the time required for maximum CL intensity. Nevertheless, a plethora of analytes have been deter mined by nonsegmented continuous-flow manifolds [2] and some of them are summarized in Table 1.
