ABSTRACT

The thermophilic archaeon Pyrococcus furiosus has a completely unique phosphoryl donor for the PFK enzyme (Kengen et al., 1996) and utilises ADP. This enzyme (ADPPFK, ADP:o-fructose-6-phosphate 1-phosphotransferase) catalyses the reaction

ADP + fructose-6-P ~ fructose-1,6-P2 +AMP

270 Thermophiles: the keys to molecular evolution and the origin of life?