ABSTRACT
Cells captured and released from the immunoaffinity devices do not have detectable mouse immunoglobulin on their surface. To determine whether residual mouse immunoglobulin present on the devices remained on the puri fied cells, isolated CD8+ and CD34+ cells were incubated with fluorochromeconjugated Abs that recognize either mouse or rabbit immunoglobulins. As measured by flow cytometry, fluorescence using either polyclonal or monoclo nal antibodies to mouse immunoglobulin always remained within the back ground staining of the antirabbit immunoglobulin Abs at 0-7% dull positive cells (n = 248, data not shown).
