ABSTRACT
Fluorescing secretory cells are clearly seen among non-secretory or secretory cells with other non-fluorescing products (Roshchina, 2003). Bright blue luminescence is observed for secretory cells of woody bud scales in birch Betula verrucosa, blue-green - for secretory hairs of potato Solanum tuberosum, glandular surface of pistil stigma of Campanula persicifolia. Microspores served for plant breeding are also secretory unicellular systems (Gimenez-Martin et al., 1969; Roshchina and Roshchina, 1989; Roshchina and Roshchina, 1993; 2003). Generative microspores from seed-breeding plants (called pollen, male gametophyte) fluoresce in different sections of the spectrum depending on the composition (Roshchina et al., 1998a; Roshchina and Melnikova, 1999). The blue-green emission is from pollen grains of birch Betula verrucosa. Spore -breeding plants have vegetative microspores, which have chloroplasts, and fluoresce (Colour Fig. 3) within the range of blue to red depending on their state. (Roshchina et al., 2002; 2003a; 2004). Root secretory cells are usually represented as secretory hairs and single cells known as idioblasts. Idioblasts of Ruta graveolens root fluoresce in the orange region of the spectrum. Confocal microscopy. Unlike a usual luminescence microscope, in confocal microscopy before being caught by a photomultiplier, the fluorescent beam from the sample studied passes through a confocal aperture called pinhole (Fig. 1.3 B). Changing the diameter of the aperture a pinhole limits the scattered light from the parts of the object outside the focal plane and contrasts it with the image (Pawley ed., 1990; Pawley and Pawley, 2006). Construction of a confocal microscope enables the observation of cellular structures by the regulation of the depth of an object slide. Laser scanning confocal microscopy (LSCM) may produce images of high quality from fluorescing cells. The images of plant secreting cells, which excrete allelochemicals, or plant cells, which serve as acceptors of allelochemicals, may be changed in allelopathic chemical relations that register by this technique.
