ABSTRACT
Standard fluorimetry. Beside the microscopy-related technique, the usual standard fluorimeter or MPF fluorescence spectrophotometer with laser 360-380 nm excitation (also in a combination with liquid nitrogen kryostation) was used for the study of the fluorescence spectra on 1-3 mm - film (layer) of pollen at room temperature and at the temperature of liquid nitrogen (Butkhuzi et a l, 2002). Due to the apparatus, more peaks in the fluorescence spectra of the samples have been observed in comparison with the spectra of pollen from the same species that was registered by microspectrofluorimetry (Roshchina et al., 1997a, b; Melnikova et al., 1997).
