ABSTRACT
The cDNA microarray and high-density cDNA analysis approaches (Bernard et al., 1996; Chen et al., 1998; DeRisi et al., 1996; Lockhart et al., 1996; Nguyen et al., 1995; Schena et al., 1995, 1996; Wodicka et al., 1997) provide efficient tools for solving the difficulties in quantifying expression of a large number of genes. By depositing hundreds or thousands of target samples, usually PCR products from cDNA, on solid substrates (glass slides or filter membranes) and hybridizing with labeled complex probes prepared from cell or tissue RNA extracts, the cDNA microarray method allows expression analysis of hundreds or thousands of genes simultaneously.
