ABSTRACT
Contents 2.1 Introduction ..................................................................................................................... 22 2.2Cultural Methods............................................................................................................ 22
2.2.1Enumeration Methods.......................................................................................... 23 2.2.1.1Plate Count............................................................................................. 23 2.2.1.2Most Probable Number.......................................................................... 24
2.2.2Detection Methods............................................................................................... 24 2.3Alternative or Rapid Microbiological Methods................................................................. 24
2.3.1Methods with a Concentration Step......................................................................25 2.3.2Detection and Enumeration Methods....................................................................25
2.4Listeria monocytogenes....................................................................................................... 27 2.4.1Detection of Listeria monocytogenes....................................................................... 27 2.4.2Enumeration of Listeria monocytogenes.................................................................. 29 2.4.3Confirmation of Listeria monocytogenes................................................................. 30
2.5Escherichia coli O157:H7................................................................................................... 32 2.5.1Detection of Escherichia coli O157:H7................................................................... 32 2.5.2Enumeration of Escherichia coli O157:H7.............................................................. 34 2.5.3Confirmation of Escherichia coli O157:H7............................................................. 34
2.6Salmonella spp...................................................................................................................35 2.6.1Detection and Confirmation of Salmonella spp..................................................... 36
2.7Staphylococcus aureus......................................................................................................... 38 2.7.1Enumeration and Confirmation of Staphylococcus aureus...................................... 38
2.8 Yersinia enterocolitica ........................................................................................................ 40 2.8.1Detection and Confirmation of Yersinia enterocolitica........................................... 40
2.9Bacillus cereus................................................................................................................... 42 2.9.1Enumeration and Confirmation of Bacillus cereus................................................. 42
2.10Clostridium perfringens..................................................................................................... 44 2.10.1Enumeration and Confirmation of Clostridium perfringens..................................45
2.11Campylobacter jejuni..........................................................................................................47 2.11.1Detection and Confirmation of Campylobacter jejuni...........................................47
References................................................................................................................................. 49
