ABSTRACT

Fluorescence-based techniques have become important tools in biomedical research and clinical medicine. A wide range of common biomedical applications are based on the measurement of ¦uorescence, including ¦uorescence microscopy, DNA sequencing, ¦ow cytometry, and ¦uorescence-based immunoassays (Lakowicz 2006). ™e use of ¦uorescence spectroscopy (FS) for biomedical diagnostics has been the subject of extensive research and has included ežorts to characterize cardiac tissue, analyze blood, and aid in screening and early detection of cancer. FS is of particular interest as a noninvasive in vivo diagnostic technique, which may provide an alternative to invasive biopsy.