ABSTRACT
Advances in the research ‚eld of bioactive proteins and peptides are driven by a molecular understanding of biological processes and various analytical techniques are a critical component of this understanding. In the past, biochemical functional analysis of peptide and protein activity was focused on the analysis of single molecules. Different up-to-date methods, including peptide synthesis and immunochemistry, have been applied to the characterization of bioactive peptides recently. Although this approach has served well, the rapid pace of discovery of new gene products by large-scale genomic and proteomic initiatives has necessitated the design of alternative strategies for analyzing protein and peptide function as compared to traditional analytical methods. Bioactive peptides have been studied by means of the
7.1 Introduction ...................................................................................................................................119 7.2 Quantitation of Peptides and Proteins .......................................................................................... 120
7.2.1 UV Light .......................................................................................................................... 120 7.2.2 Lowry Method ................................................................................................................. 120 7.2.3 Bicinchoninic Assay .........................................................................................................121 7.2.4 Bradford Method ..............................................................................................................121 7.2.5 Dumas Method .................................................................................................................121
