ABSTRACT
Electrochemical biosensors are usually based on redox reactions
that consume or produce electrons. Such a device can be re-
presented by an indication electrode, which integrates receptor-
transducer element providing selective quantitative analytical infor-
mation; recorded signals are proportional to analyte concentrations.
There are several types of electrochemical transducers, from which
amperometric transducers are most used in biosensors due to
their high sensitivity and selectivity. Except an indication electrode,
the electrochemical system contains other two electrodes, that
is, a reference electrode and an auxiliary electrode [1-4]. Our
approach in electrochemical oligonucleotide (ODN) transducer is
built on the adsorptive stripping voltammetric (AdSV) technique
in connection with elimination voltammetry with linear scan
(EVLS). Generally, EVLS enables the elimination of selected partial
voltammetric currents and the conservation of the other one
contributing to the increase of current sensitivity, the expansion of
electrode potential range (potential window) and the separation of
overlapped voltammetric signals. The basic idea of EVLS procedure
lies in the different dependencies of various voltammetric current
components on the scan rate. The elimination result can be achieved
by a function obtained by linear combination of total voltammetric
currents measured at different scan rates [5, 6].