Rice seeds of BRRI dhan29 were collected from Bangladesh Rice Research Institute (BRRI), Bangladesh. The seeds were surface-sterilized using 1% methyl-1-butylcarbamoyl-2-benzimidazole carbonate solution. Fe concentrations in the MS pre-experimental and experimental solutions were 0.03 and 0.36 mM, respectively. The pHs of the pre-experimental and experimental solutions were adjusted to 6.5 and 9, respectively. Solutions of EDDS, HIDS, and MGDA at desired concentrations were prepared from ethylenediaminedisuccinic acid (Chelest corporation, Japan), tetrasodium hydroxyiminodisuccinate (Nippon Syokubai, Japan), and methylglycin diacetic acid (BASF, USA), respectively. Other reagents were of analytical grade or better. All solutions were prepared with DI water.