ABSTRACT
For the interaction studies, rats were given a single dose of As at 2.5 mg/kg (oral gavage) as sodium arsenate immediately followed by Cd at 3 or 6 mg/kg (oral gavage) as cadmium chloride solution. Urine and blood were collected as described above. As and Cd concentrations were measured by ICP-MS (Agilent 7500 CS) together with appropriate Certified Reference Materials (CRMs) for quality control of blood (Seronorm, Level2, Sero As), urine (Lyphocheck, level1, Bio-Rad, California, USA) and tissue samples (SRM 1577b, freeze dried bovine liver). Absolute Bioavailability (ABA) of As was calculated from the area under the curve of either urine or blood compared to that of the intravenous positive control group using Graphpad Prism software (Version 5, San Diego, USA). PKSolver was used to analyze pharmacokinetic parameters using concentration-time data (Zhang et al. 2010).
