ABSTRACT
The cells were grown in 6-well culture dishes and exposed to As3+ (10 μM) with or without choloroquine (50 μM) or cytochalasin D (1 μg/mL). After washing the monolayer 3 times with Phosphate Buffered Saline (PBS), 0.75 mL of nitric acid and 0.25 mL of hydrogen peroxide were added to each well and the dishes were incubated overnight at room temperature. The solution was transferred to an acid-washed test tube and was wet-digested at 130°C for 2 d in an aluminum dry block bath. The samples were diluted with deionizer water and arsenic concentrations were measured by inductively coupled plasma mass spectrometry (ICPMS, HP7500cs, Yokokawa Analytical Systems, Tokyo).
2.3 Western blotting
