ABSTRACT
Conclusion 154 References 154
6E Crystallography, nuclear magnetic resonance, and modeling 157 Introduction 157 X-ray crystallography 158 Nuclear magnetic resonance spectroscopy 163 Further techniques for probing protein structure 165 Molecular modeling 165 Structural genomics 166 References 166
6F Functional analysis of mutant proteins in cells 169 Introduction 169 Techniques for expressing proteins in cells 169 Mechanisms of receptor synthesis and trafficking 169 Receptor binding, internalization, and cycling
within cells 170 Analysis of cellular receptor localization
and trafficking 170 Analyzing ligand binding to cell surface receptors 172 Examining receptor-mediated intracellular
signaling pathways 172 References 174
