ABSTRACT

QC 4.4.3. Each batch of dilution=rinse water should be checked for sterility by adding 50mL of water to 50mL of a double strength nonselective broth (e.g. tryptic soy, trypticase soy, or tryptose broth). Incubate at 35 0.5C for 24 hr and check for growth. Discard if growth is detected.